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Microbiome 16S Amplification & Sequencing

Quickly monitor bacterial communities with full-length 16S sequencing.

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Quickly monitor bacterial communities with full-length 16S sequencing.

Order Now

Quickly monitor bacterial communities with full-length 16S sequencing.

Order Now

Microbiome 16S Amplification & Sequencing

Quickly monitor bacterial communities with full-length 16S sequencing.

Order Now

Microbiome 16S Amplification & Sequencing

Quickly monitor bacterial communities with full-length 16S sequencing.

Order Now

See sample prep and shipping instructions

16S Amplification & Sequencing without extraction

16S Amplification & Sequencing with extraction

How It Works
Preparing Your Metagenomic DNA Samples

Step 1

Prepare your samples

Send us purified genomic DNA that has been extracted at your lab from the source material (soil, swabs, feces. etc.).

Submit your final purified metagenomic DNA normalized to 10ng/uL in elution buffer (10 mM Tris, pH 8.5) or nuclease-free water.

Send us purified genomic DNA that has been extracted at your lab from the source material (soil, swabs, feces. etc.).

Submit your final purified metagenomic DNA normalized to 10ng/uL in elution buffer (10 mM Tris, pH 8.5) or nuclease-free water.

Send us purified genomic DNA that has been extracted at your lab from the source material (soil, swabs, feces. etc.).

Submit your final purified metagenomic DNA normalized to 10ng/uL in elution buffer (10 mM Tris, pH 8.5) or nuclease-free water.

Step 2

Place your order and drop off your samples

Place your order online, then drop off your sample at a dropbox or ship it with a printed label.

Step 3

Get results overnight

The next morning you’ll receive an email to download your results from your Dashboard.

How it works

Preparing Your Biological Source Material

Step 1

Prepare your source material

Gather the specified weight or volume of your sample.

Gather the specified weight or volume of your sample.

Gather the specified weight or volume of your sample.

Add 500 uL of Zymo 1X DNA/RNA Shield into a 2 mL screw cap tube.
Place the specified amount of the source material into the tube containing DNA/RNA Shield. Invert 5-10x to mix.

Add 500 uL of Zymo 1X DNA/RNA Shield into a 2 mL screw cap tube.
Place the specified amount of the source material into the tube containing DNA/RNA Shield. Invert 5-10x to mix.

Add 500 uL of Zymo 1X DNA/RNA Shield into a 2 mL screw cap tube.
Place the specified amount of the source material into the tube containing DNA/RNA Shield. Invert 5-10x to mix.

Step 2

Place your order online and send using the printed label

Label each screw cap tube with the first two characters of the order code and the sample number. For example, “XX-3” would indicate order XXYYZZ, sample 3.
Wrap 2 mL screw cap tubes in foil.
Place the foil wrapped screw cap tubes in a rigid container, such as a falcon tube or tube box.
Double-bag the rigid vessel in at least two plastic bags.
Place an absorbent pad into the plastic bag along with the rigid vessel. For orders of more than 10 samples, please put the tubes in a tube box and load the samples row by row in numerical order.
Ship samples using the printed label directly to our Eugene, OR lab at room temperature. Dry ice and cold packs are not necessary.

Ship to:

Plasmidsaurus
1850 Millrace Drive, Suite 200
Eugene, OR 97403

Label each screw cap tube with the first two characters of the order code and the sample number. For example, “XX-3” would indicate order XXYYZZ, sample 3.
Wrap 2 mL screw cap tubes in foil.
Place the foil wrapped screw cap tubes in a rigid container, such as a falcon tube or tube box.
Double-bag the rigid vessel in at least two plastic bags.
Place an absorbent pad into the plastic bag along with the rigid vessel. For orders of more than 10 samples, please put the tubes in a tube box and load the samples row by row in numerical order.
Ship samples using the printed label directly to our Eugene, OR lab at room temperature. Dry ice and cold packs are not necessary.

Ship to:

Plasmidsaurus
1850 Millrace Drive, Suite 200
Eugene, OR 97403

Label each screw cap tube with the first two characters of the order code and the sample number. For example, “XX-3” would indicate order XXYYZZ, sample 3.
Wrap 2 mL screw cap tubes in foil.
Place the foil wrapped screw cap tubes in a rigid container, such as a falcon tube or tube box.
Double-bag the rigid vessel in at least two plastic bags.
Place an absorbent pad into the plastic bag along with the rigid vessel. For orders of more than 10 samples, please put the tubes in a tube box and load the samples row by row in numerical order.
Ship samples using the printed label directly to our Eugene, OR lab at room temperature. Dry ice and cold packs are not necessary.

Ship to:

Plasmidsaurus
1850 Millrace Drive, Suite 200
Eugene, OR 97403

Place your order online, then drop off your sample at a dropbox or ship it with a printed label.

Step 3

Get results in 3 business days or less

You’ll receive an email to download your results from your Dashboard.

Step 1

Prepare your samples

Gather the specified weight or volume of your sample.

Add 500 uL of Zymo 1X DNA/RNA Shield into a 2 mL screw cap tube.
Place the specified amount of the source material into the tube containing DNA/RNA Shield. Invert 5-10x to mix.

Step 2

Place your order online and send using the printed label

Label each screw cap tube with the first two characters of the order code and the sample number. For example, “XX-3” would indicate order XXYYZZ, sample 3.
Wrap 2 mL screw cap tubes in foil.
Place the foil wrapped screw cap tubes in a rigid container, such as a falcon tube or tube box.
Double-bag the rigid vessel in at least two plastic bags.
Place an absorbent pad into the plastic bag along with the rigid vessel. For orders of more than 10 samples, please put the tubes in a tube box and load the samples row by row in numerical order.
Ship samples using the printed label directly to our Eugene, OR lab at room temperature. Dry ice and cold packs are not necessary.

Ship to:

Plasmidsaurus
1850 Millrace Drive, Suite 200
Eugene, OR 97403

Step 3

Get results in 3 business days or less

You’ll receive an email to download your results from your Dashboard.

Data and File Types Delivered

Raw read sequences (.fastq.gz file)

Data and File Types Delivered

Provides the sequences of all raw reads produced by your sample (up to 5,000 raw reads for Standard service, 10,000 raw reads for Big, 20,000 raw reads for Huge, and 500,000 raw reads for Bronto).

Raw read sequences (.fastq.gz file)

Provides the sequences of all raw reads produced by your sample (up to 5,000 raw reads for Standard service, 10,000 raw reads for Big, 20,000 raw reads for Huge, and 500,000 raw reads for Bronto).

Raw read sequences (.fastq.gz file)

Provides the sequences of all raw reads produced by your sample (up to 5,000 raw reads for Standard service, 10,000 raw reads for Big, 20,000 raw reads for Huge, and 500,000 raw reads for Bronto).

Taxonomy table (.csv file)

Lists all taxa found in your sample that are above a relative threshold of 0.1% abundance. The list includes columns with the NCBI taxonomy ID, relative abundance, and full taxonomic ranks for each taxa from kingdom to species.

Stats (.csv file)

Lists statistics about the sequencing reads (total number reads, total number bases, read quality, etc.)

Taxonomy plot by genus (.png file)

Shows the relative abundance of the top 20 genera in your sample.

Taxonomy plot by species (.png file)

Shows the relative abundance of the top 20 species in your sample.

Read length histogram (.png file)

Displays the read lengths of all raw reads produced by the sample, including coloration to indicate the kingdom that the reads originate from (bacteria, archaea, protists, fungi, etc.)

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FAQ

How do I ensure the best chance of successful sequencing?

I have a unique raw biological sample type, can you perform the DNA extraction for me?

Why is extraction only available to US-based customers?

Can I use my local US dropbox to ship raw biological materials?

I already have amplicons prepared from the 16S, 18S, and/or ITS genes. Which Microbiome service should I use?

Where can I find general information about all Microbiome Sequencing services?

How do you sequence 16S Amplification & Sequencing samples?

Do I need to ship any primers with my sample?

How is taxonomic analysis performed for Microbiome Amplicon samples?

What data & file types will I receive for successful 16S Amplification & Sequencing sequencing?

How much sequencing coverage will I get for 16S Amplification & Sequencing samples?

What is the limit of detection for the lowest abundance community members that can be detected with the 16S Amplification & Sequencing service?

How accurate are the 16S Amplification & Sequencing results?

What is your turnaround time for returning 16S Amplification & Sequencing results?

How do I get my results?

How do I extract and purify metagenomic DNA for the 16S Amplification & Sequencing service?

How do I quantify my purified metagenomic DNA?

How do I measure the quality and purity of my purified metagenomic DNA?

What guarantees do you provide?

Why did my 16S Amplification & Sequencing sample fail?

What is your policy when 16S Amplification & Sequencing samples fail?

Can you perform whole-genome sequencing and assembly of my metagenomic gDNA?

I have suggestions about the types of reports and figures you should generate from microbial amplicon results, or which databases you should use for processing the data. How can I let you know?

How It Works
Preparing Your Metagenomic DNA Samples

Step 1

Prepare your samples

Send us purified genomic DNA that has been extracted at your lab from the source material (soil, swabs, feces. etc.).

Submit your final purified metagenomic DNA normalized to 10ng/uL in elution buffer (10 mM Tris, pH 8.5) or nuclease-free water.

Step 2

Place your order online, then drop off your sample at a dropbox or ship it with a printed label.

Place your order and drop off your samples

Step 3

Get results overnight

The next morning you’ll receive an email to download your results from your Dashboard.

See sample prep and shipping instructions

16S Amplification & Sequencing without extraction

16S Amplification & Sequencing with extraction

See sample prep and shipping instructions

16S Amplification & Sequencing without extraction

16S Amplification & Sequencing with extraction

Ready to get started?

Order Now

How It Works Preparing Your Metagenomic DNA Samples

How It Works Preparing Your Metagenomic DNA Samples

How It Works Preparing Your Metagenomic DNA Samples

How It Works Preparing Your Metagenomic DNA Samples

How it works

Preparing Your Biological Source Material

Preparing Your Biological Source Material

Preparing Your Biological Source Material

Preparing Your Biological Source Material

Data and File Types Delivered

Data and File Types Delivered

Raw read sequences (.fastq.gz file)

Data and File Types Delivered

Data and File Types Delivered

Provides the sequences of all raw reads produced by your sample (up to 5,000 raw reads for Standard service, 10,000 raw reads for Big, 20,000 raw reads for Huge, and 500,000 raw reads for Bronto).

Raw read sequences (.fastq.gz file)

Provides the sequences of all raw reads produced by your sample (up to 5,000 raw reads for Standard service, 10,000 raw reads for Big, 20,000 raw reads for Huge, and 500,000 raw reads for Bronto).

Raw read sequences (.fastq.gz file)

Provides the sequences of all raw reads produced by your sample (up to 5,000 raw reads for Standard service, 10,000 raw reads for Big, 20,000 raw reads for Huge, and 500,000 raw reads for Bronto).

Taxonomy table (.csv file)

Taxonomy table (.csv file)

Lists all taxa found in your sample that are above a relative threshold of 0.1% abundance. The list includes columns with the NCBI taxonomy ID, relative abundance, and full taxonomic ranks for each taxa from kingdom to species.

Stats (.csv file)

Stats (.csv file)

Stats (.csv file)

Lists statistics about the sequencing reads (total number reads, total number bases, read quality, etc.)

Taxonomy plot by genus (.png file)

Taxonomy plot by genus (.png file)

Taxonomy plot by genus (.png file)

Shows the relative abundance of the top 20 genera in your sample.

Taxonomy plot by species (.png file)

Taxonomy plot by species (.png file)

Taxonomy plot by species (.png file)

Shows the relative abundance of the top 20 species in your sample.

Read length histogram (.png file)

Read length histogram (.png file)

Read length histogram (.png file)

Displays the read lengths of all raw reads produced by the sample, including coloration to indicate the kingdom that the reads originate from (bacteria, archaea, protists, fungi, etc.)

FAQ

FAQ

FAQ

FAQ

How It Works Preparing Your Metagenomic DNA Samples

Ready to get started?

Ready to get started?

Ready to get started?

Ready to get started?

How It Works
Preparing Your Metagenomic DNA Samples

How It Works
Preparing Your Metagenomic DNA Samples

How It Works
Preparing Your Metagenomic DNA Samples

How It Works
Preparing Your Metagenomic DNA Samples

How It Works
Preparing Your Metagenomic DNA Samples