See sample prep and shipping instructions
See sample prep and shipping instructions
Complete Coverage
Resolve entire bacterial genomes with long-read sequencing that captures plasmids, repeats, and structural variants.
Resolve entire bacterial genomes with long-read sequencing that captures plasmids, repeats, and structural variants.
Resolve entire bacterial genomes with long-read sequencing that captures plasmids, repeats, and structural variants.
Rapid and Flexible
Get a high-accuracy genome assembly in 1 day from extracted DNA, or in about 2 days from cell pellets.
Get a high-accuracy genome assembly in 1 day from extracted DNA, or in about 2 days from cell pellets.
Get a high-accuracy genome assembly in 1 day from extracted DNA, or in about 2 days from cell pellets.
Comprehensive Data
Receive a fully annotated genome assembly, raw sequencing data, and support from our scientists.
Receive a fully annotated genome assembly, raw sequencing data, and support from our scientists.
Receive a fully annotated genome assembly, raw sequencing data, and support from our scientists.
Complete Coverage
Resolve entire bacterial genomes with long-read sequencing that captures plasmids, repeats, and structural variants.
Rapid and Flexible
Get a high-accuracy genome assembly in 1 day from extracted DNA, or in about 2 days from cell pellets.
Comprehensive Data
Receive a fully annotated genome assembly, raw sequencing data, and support from our scientists.
Most bacterial genome sequencing still relies on short reads and slow turnaround times, often leaving assemblies fragmented or incomplete. Plasmidsaurus Bacterial Genome Sequencing gives you the full picture, fast. Designed for fast, complete assemblies, this service is ideal for tracking antimicrobial resistance genes, identifying engineered strains, and validating reference genomes.
We generate complete, de novo bacterial assemblies from clonal samples which capture large insertions and structural variants. We collect raw reads from chromosomal, plasmid, and phage DNA, giving you a full picture of your bug’s genome. You can send purified genomic DNA or bacterial cell pellets.
An optional Hybrid workflow adds short-read Illumina sequencing data for applications that require ultra-precise SNP detection.
Most bacterial genome sequencing still relies on short reads and slow turnaround times, often leaving assemblies fragmented or incomplete. Plasmidsaurus Bacterial Genome Sequencing gives you the full picture, fast. Designed for fast, complete assemblies, this service is ideal for tracking antimicrobial resistance genes, identifying engineered strains, and validating reference genomes.
We generate complete, de novo bacterial assemblies from clonal samples which capture large insertions and structural variants. We collect raw reads from chromosomal, plasmid, and phage DNA, giving you a full picture of your bug’s genome. You can send purified genomic DNA or bacterial cell pellets.
An optional Hybrid workflow adds short-read Illumina sequencing data for applications that require ultra-precise SNP detection.
Data and File Types Delivered
Data and File Types Delivered
When results are ready, you’ll receive an email to log in and download from your Dashboard.
Raw read sequences (.fastq.gz file)
Data and File Types Delivered
Provides the sequences of all reads produced by your sample from chromosomes, plasmids, and phage DNA.
Raw read sequences (.fastq.gz file)
Provides the sequences of all reads produced by your sample from chromosomes, plasmids, and phage DNA.
Annotated assembled sequences (.gbff, .gbk, .embl files)
Annotated assembled sequences (.gbff, .gbk, .embl files)
Summarizes the polished and annotated consensus sequence of the genome.
Feature sequences (.fna, .ffn, .faa files)
Feature sequences (.fna, .ffn, .faa files)
Lists in fasta formats sequences including whole contigs (.fna), gene sequences (.ffn), and translated protein amino acid sequences (.faa).
Feature annotation evidence (.gff, .tsv, .txt, .json files)
Feature annotation evidence (.gff, .tsv, .txt, .json files)
Details the annotations made by Bakta in multiple formats, separating high-confidence annotations from hypotheticals.
Taxonomical assignment (.tsv, .txt, .png files)
Taxonomical assignment (.tsv, .txt, .png files)
Shows the taxonomical assignment of each contig with the best-match reference and degree of similarity.
Summary report (.html, .png, .tsv, .txt files)
Summary report (.html, .png, .tsv, .txt files)
An analytical report displaying all the figures and the key metrics for the assembly, annotation, and species assignment. Includes a circos plot, read quality plot and statistics, Bandage assembly graph, and species assignment statistics.
How it works
How it works
Preparing your gDNA samples
Preparing your gDNA samples
Preparing your gDNA samples
Step 1
Prepare your samples
Prepare your samples
Prepare your samples
Extract and purify gDNA from your clonal bacterial culture.
Perform QC to verify your gDNA quantity, quality, and purity. We recommend >50% of the DNA is above 15 kb in length with a purity of 260/280 above 1.8 and 260/230 between 2.0-2.2.
Send us your gDNA normalized to 50 ng/uL in 20 uL of elution buffer (10 mM Tris, pH 8.5).
Note: For our Hybrid Sequencing service, send your gDNA normalized to 50 ng/uL in 30 uL of elution buffer (10 mM Tris, pH 8.5)
Step 2
Place your order and drop off your samples
Place your order and drop off your samples
Place your order online.
Label and package your extracted gDNA per our standard shipping instructions.
Then drop off your sample at a dropbox or ship it with a printed label.
Step 3
Get results fast
Get results fast
You’ll receive an email to download your results from your Dashboard.
Step 1
Prepare your samples
Extract and purify gDNA from your clonal bacterial culture.
Perform QC to verify your gDNA quantity, quality, and purity. We recommend >50% of the DNA is above 15 kb in length with a purity of 260/280 above 1.8 and 260/230 between 2.0-2.2.
Send us your gDNA normalized to 50 ng/uL in 20 uL of elution buffer (10 mM Tris, pH 8.5).
Note: For our Hybrid Sequencing service, send your gDNA normalized to 50 ng/uL in 30 uL of elution buffer (10 mM Tris, pH 8.5)
Step 2
Place your order and drop off your samples
Place your order online.
Label and package your extracted gDNA per our standard shipping instructions.
Then drop off your sample at a dropbox or ship it with a printed label.
How it works
How it works
Preparing your bacterial cell pellets
Preparing your bacterial cell pellets
Preparing your bacterial cell pellets
Step 1
Prepare your samples
Prepare your samples
Prepare your samples
All cell pellets must be counted to ensure you are sending 4-6 x 10^9 cells and then resuspended in 500 µL of Zymo 1X DNA/RNA Shield preservative in a 2 mL screw cap tube. Please do not use any other type of preservation media. We accept cell pellets from both BSL1 and BSL2 strains.
Step 2
Place your order and drop off your samples
Place your order and drop off your samples
Place your order online.
Label each 2 mL screw cap tube with the first two digits of your order code, sample number, and a plus (+) or minus (-) to indicate whether each strain is gram positive or gram negative.
Then drop off your sample at a dropbox or ship it with a printed label.
Step 3
Get results fast
Get results fast
You’ll receive an email to download your results from your Dashboard.
Step 1
Prepare your samples
All cell pellets must be counted to ensure you are sending 4-6 x 10^9 cells and then resuspended in 500 µL of Zymo 1X DNA/RNA Shield preservative in a 2 mL screw cap tube. Please do not use any other type of preservation media. We accept cell pellets from both BSL1 and BSL2 strains.
Step 2
Place your order and drop off your samples
Place your order online.
Label each 2 mL screw cap tube with the first two digits of your order code, sample number, and a plus (+) or minus (-) to indicate whether each strain is gram positive or gram negative.
Then drop off your sample at a dropbox or ship it with a printed label.
FAQ
FAQ
How do you sequence bacterial genome samples?
How do you sequence bacterial genome samples?
How do you sequence bacterial genome samples?
How is the bacterial genome assembly generated?
How is the bacterial genome assembly generated?
How is the bacterial genome assembly generated?
What data & file types will I receive for successful bacterial genome sequencing?
What data & file types will I receive for successful bacterial genome sequencing?
What data & file types will I receive for successful bacterial genome sequencing?
Can you identify the species if I don’t know it beforehand?
Can you identify the species if I don’t know it beforehand?
Can you identify the species if I don’t know it beforehand?
Will I get a fully closed genome?
Will I get a fully closed genome?
Will I get a fully closed genome?
Is raw data included?
Is raw data included?
Is raw data included?
Can you confirm CRISPR edits or gene placements?
Can you confirm CRISPR edits or gene placements?
Can you confirm CRISPR edits or gene placements?
How do I get my results?
How do I get my results?
How do I get my results?
What data targets do you use for the Oxford Nanopore sequencing service? What determines the “complete” and “fail” sequencing statuses?
What data targets do you use for the Oxford Nanopore sequencing service? What determines the “complete” and “fail” sequencing statuses?
What data targets do you use for the Oxford Nanopore sequencing service? What determines the “complete” and “fail” sequencing statuses?
What data targets do you use for the Illumina portion of the Hybrid (Oxford Nanopore + Illumina) sequencing service? What determines the “complete” and “fail” sequencing statuses?
What data targets do you use for the Illumina portion of the Hybrid (Oxford Nanopore + Illumina) sequencing service? What determines the “complete” and “fail” sequencing statuses?
What data targets do you use for the Illumina portion of the Hybrid (Oxford Nanopore + Illumina) sequencing service? What determines the “complete” and “fail” sequencing statuses?
What are the benefits of the Hybrid (Oxford Nanopore + Illumina) sequencing service?
What are the benefits of the Hybrid (Oxford Nanopore + Illumina) sequencing service?
What are the benefits of the Hybrid (Oxford Nanopore + Illumina) sequencing service?
How do I prepare a clonal culture of bacteria?
How do I prepare a clonal culture of bacteria?
How do I prepare a clonal culture of bacteria?
How do I extract and purify gDNA from my clonal bacterial culture?
How do I extract and purify gDNA from my clonal bacterial culture?
How do I extract and purify gDNA from my clonal bacterial culture?
How do I prepare my bacterial cell pellets for extraction?
How do I prepare my bacterial cell pellets for extraction?
How do I prepare my bacterial cell pellets for extraction?
Does your extraction service include growing cultures prior to extraction?
Does your extraction service include growing cultures prior to extraction?
Does your extraction service include growing cultures prior to extraction?
Do you perform any QC on my gDNA prior to sequencing?
Do you perform any QC on my gDNA prior to sequencing?
Do you perform any QC on my gDNA prior to sequencing?
How should I QC my gDNA prior to submitting my sample?
How should I QC my gDNA prior to submitting my sample?
How should I QC my gDNA prior to submitting my sample?
How should I handle gDNA samples?
How should I handle gDNA samples?
How should I handle gDNA samples?
What guarantees do you provide?
What guarantees do you provide?
What guarantees do you provide?
What is your policy when bacterial genome sequencing fails?
What is your policy when bacterial genome sequencing fails?
What is your policy when bacterial genome sequencing fails?
FAQ
How do you sequence bacterial genome samples?
How do you sequence bacterial genome samples?
How is the bacterial genome assembly generated?
How is the bacterial genome assembly generated?
What data & file types will I receive for successful bacterial genome sequencing?
What data & file types will I receive for successful bacterial genome sequencing?
Can you identify the species if I don’t know it beforehand?
Can you identify the species if I don’t know it beforehand?
Will I get a fully closed genome?
Will I get a fully closed genome?
Is raw data included?
Is raw data included?
Can you confirm CRISPR edits or gene placements?
Can you confirm CRISPR edits or gene placements?
How do I get my results?
How do I get my results?
What data targets do you use for the Oxford Nanopore sequencing service? What determines the “complete” and “fail” sequencing statuses?
What data targets do you use for the Oxford Nanopore sequencing service? What determines the “complete” and “fail” sequencing statuses?
What data targets do you use for the Illumina portion of the Hybrid (Oxford Nanopore + Illumina) sequencing service? What determines the “complete” and “fail” sequencing statuses?
What data targets do you use for the Illumina portion of the Hybrid (Oxford Nanopore + Illumina) sequencing service? What determines the “complete” and “fail” sequencing statuses?
What are the benefits of the Hybrid (Oxford Nanopore + Illumina) sequencing service?
What are the benefits of the Hybrid (Oxford Nanopore + Illumina) sequencing service?
How do I prepare a clonal culture of bacteria?
How do I prepare a clonal culture of bacteria?
How do I extract and purify gDNA from my clonal bacterial culture?
How do I extract and purify gDNA from my clonal bacterial culture?
How do I prepare my bacterial cell pellets for extraction?
How do I prepare my bacterial cell pellets for extraction?
Does your extraction service include growing cultures prior to extraction?
Does your extraction service include growing cultures prior to extraction?
Do you perform any QC on my gDNA prior to sequencing?
Do you perform any QC on my gDNA prior to sequencing?
How should I QC my gDNA prior to submitting my sample?
How should I QC my gDNA prior to submitting my sample?
How should I handle gDNA samples?
How should I handle gDNA samples?
What guarantees do you provide?
What guarantees do you provide?
What is your policy when bacterial genome sequencing fails?
What is your policy when bacterial genome sequencing fails?
Most bacterial genome sequencing still relies on short reads and slow turnaround times, often leaving assemblies fragmented or incomplete. Plasmidsaurus Bacterial Genome Sequencing gives you the full picture, fast. Designed for fast, complete assemblies, this service is ideal for tracking antimicrobial resistance genes, identifying engineered strains, and validating reference genomes.
We generate complete, de novo bacterial assemblies from clonal samples which capture large insertions and structural variants. We collect raw reads from chromosomal, plasmid, and phage DNA, giving you a full picture of your bug’s genome. You can send purified genomic DNA or bacterial cell pellets.
An optional Hybrid workflow adds short-read Illumina sequencing data for applications that require ultra-precise SNP detection.
Most bacterial genome sequencing still relies on short reads and slow turnaround times, often leaving assemblies fragmented or incomplete. Plasmidsaurus Bacterial Genome Sequencing gives you the full picture, fast. Designed for fast, complete assemblies, this service is ideal for tracking antimicrobial resistance genes, identifying engineered strains, and validating reference genomes.
We generate complete, de novo bacterial assemblies from clonal samples which capture large insertions and structural variants. We collect raw reads from chromosomal, plasmid, and phage DNA, giving you a full picture of your bug’s genome. You can send purified genomic DNA or bacterial cell pellets.
An optional Hybrid workflow adds short-read Illumina sequencing data for applications that require ultra-precise SNP detection.
Most bacterial genome sequencing still relies on short reads and slow turnaround times, often leaving assemblies fragmented or incomplete. Plasmidsaurus Bacterial Genome Sequencing gives you the full picture, fast. Designed for fast, complete assemblies, this service is ideal for tracking antimicrobial resistance genes, identifying engineered strains, and validating reference genomes.
We generate complete, de novo bacterial assemblies from clonal samples which capture large insertions and structural variants. We collect raw reads from chromosomal, plasmid, and phage DNA, giving you a full picture of your bug’s genome. You can send purified genomic DNA or bacterial cell pellets.
An optional Hybrid workflow adds short-read Illumina sequencing data for applications that require ultra-precise SNP detection.
See sample prep and shipping instructions