Get complete, high-quality bacterial genome assemblies with rapid turnaround. We deliver a fully annotated genome plus all raw reads, giving you confidence and control over your research.
Get complete, high-quality bacterial genome assemblies with rapid turnaround. We deliver a fully annotated genome plus all raw reads, giving you confidence and control over your research.
Get complete, high-quality bacterial genome assemblies with rapid turnaround. We deliver a fully annotated genome plus all raw reads, giving you confidence and control over your research.
Comprehensive & Accurate
Our long read sequencing resolves entire bacterial genomes, capturing plasmids, repeats, antimicrobial resistance (AMR) genes, and structural variants in contiguous, high-quality reads.
Our long read sequencing resolves entire bacterial genomes, capturing plasmids, repeats, antimicrobial resistance (AMR) genes, and structural variants in contiguous, high-quality reads.
Our long read sequencing resolves entire bacterial genomes, capturing plasmids, repeats, antimicrobial resistance (AMR) genes, and structural variants in contiguous, high-quality reads.
Rapid Results
Get your results in just a few days. We can sequence directly from your cell pellet, so there’s no need to isolate gDNA before shipping.
Get your results in just a few days. We can sequence directly from your cell pellet, so there’s no need to isolate gDNA before shipping.
Get your results in just a few days. We can sequence directly from your cell pellet, so there’s no need to isolate gDNA before shipping.
Comprehensive Data
Receive a fully annotated genome assembly, raw sequencing data, and support from our team of scientists. All of your results are delivered right to your inbox.
Receive a fully annotated genome assembly, raw sequencing data, and support from our team of scientists. All of your results are delivered right to your inbox.
Receive a fully annotated genome assembly, raw sequencing data, and support from our team of scientists. All of your results are delivered right to your inbox.
Comprehensive & Accurate
Our long read sequencing resolves entire bacterial genomes, capturing plasmids, repeats, antimicrobial resistance (AMR) genes, and structural variants in contiguous, high-quality reads.
Rapid Results
Get your results in just a few days. We can sequence directly from your cell pellet, so there’s no need to isolate gDNA before shipping.
Comprehensive Data
Receive a fully annotated genome assembly, raw sequencing data, and support from our team of scientists. All of your results are delivered right to your inbox.
Product Information
Product Information
Plasmidsaurus Bacterial Genome Sequencing is intended for whole-genome sequencing and assembly of genomic DNA (gDNA) from a clonal bacterial population (single species). You can send us pre-extracted gDNA for the fastest turnaround, or rely on our in-house extraction for added convenience.
Because we rely on long-read sequencing, our bacterial genome assemblies frequently come out fully or near-fully closed, ensuring a more complete picture of your organism.
Plasmidsaurus Bacterial Genome Sequencing is intended for whole-genome sequencing and assembly of genomic DNA (gDNA) from a clonal bacterial population (single species). You can send us pre-extracted gDNA for the fastest turnaround, or rely on our in-house extraction for added convenience.
Because we rely on long-read sequencing, our bacterial genome assemblies frequently come out fully or near-fully closed, ensuring a more complete picture of your organism.
Key Benefits
Fast Turnaround
• 1-day TAT saves days vs. competitor offerings. • Compress multiple strain engineering cycles into a single workweek.
Extraction-Free Workflow
• Sequence directly from bacterial cell pellets—no need to isolate gDNA. • Streamlines high-throughput screens, saving labor hours.
Hybrid Option (ONT + Illumina) for Highest Accuracy
• SNP Detection: The polished hybrid assembly data can be aligned to your reference to accurately identify mutations. • Resolve Complex Regions: Polish out homopolymer and methylation motif errors in ONT assemblies for maximum accuracy.
Key Benefits
Fast Turnaround
• 1-day TAT saves days vs. competitor offerings. • Compress multiple strain engineering cycles into a single workweek.
Extraction-Free Workflow
• Sequence directly from bacterial cell pellets—no need to isolate gDNA. • Streamlines high-throughput screens, saving labor hours.
Hybrid Option (ONT + Illumina) for Highest Accuracy
• SNP Detection: The polished hybrid assembly data can be aligned to your reference to accurately identify mutations. • Resolve Complex Regions: Polish out homopolymer and methylation motif errors in ONT assemblies for maximum accuracy.
Key Benefits
Fast Turnaround
• 1-day TAT saves days vs. competitor offerings. • Compress multiple strain engineering cycles into a single workweek.
Extraction-Free Workflow
• Sequence directly from bacterial cell pellets—no need to isolate gDNA. • Streamlines high-throughput screens, saving labor hours.
Hybrid Option (ONT + Illumina) for Highest Accuracy
• SNP Detection: The polished hybrid assembly data can be aligned to your reference to accurately identify mutations. • Resolve Complex Regions: Polish out homopolymer and methylation motif errors in ONT assemblies for maximum accuracy.
Key Benefits
Fast Turnaround
• 1-day TAT saves days vs. competitor offerings. • Compress multiple strain engineering cycles into a single workweek.
Extraction-Free Workflow
• Sequence directly from bacterial cell pellets—no need to isolate gDNA. • Streamlines high-throughput screens, saving labor hours.
Hybrid Option (ONT + Illumina) for Highest Accuracy
• SNP Detection: The polished hybrid assembly data can be aligned to your reference to accurately identify mutations. • Resolve Complex Regions: Polish out homopolymer and methylation motif errors in ONT assemblies for maximum accuracy.
Key Benefits
Fast Turnaround
• 1-day TAT saves days vs. competitor offerings. • Compress multiple strain engineering cycles into a single workweek.
Extraction-Free Workflow
• Sequence directly from bacterial cell pellets—no need to isolate gDNA. • Streamlines high-throughput screens, saving labor hours.
Hybrid Option (ONT + Illumina) for Highest Accuracy
• SNP Detection: The polished hybrid assembly data can be aligned to your reference to accurately identify mutations. • Resolve Complex Regions: Polish out homopolymer and methylation motif errors in ONT assemblies for maximum accuracy.
Turnaround Times & Workflow Options
Pre-Extracted gDNA
1–2 business days from receipt of samples. Ideal if you already have purified, high-quality DNA.
In-House gDNA Extraction
3–5 business days if we extract DNA from your bacterial cell pellet.
Hybrid Sequencing (ONT + Illumina)
• 6–8 business days for hybrid assembly, or 8–10 business days if we also perform in-house DNA extraction. • Perfect for applications requiring ultra-accurate SNP detection or polished assemblies to resolve tricky homopolymers.
Turnaround Times & Workflow Options
Pre-Extracted gDNA
1–2 business days from receipt of samples. Ideal if you already have purified, high-quality DNA.
In-House gDNA Extraction
3–5 business days if we extract DNA from your bacterial cell pellet.
Hybrid Sequencing (ONT + Illumina)
• 6–8 business days for hybrid assembly, or 8–10 business days if we also perform in-house DNA extraction. • Perfect for applications requiring ultra-accurate SNP detection or polished assemblies to resolve tricky homopolymers.
Turnaround Times & Workflow Options
Pre-Extracted gDNA
1 business day from receipt of samples. Ideal if you already have purified, high-quality DNA.
In-House gDNA Extraction
1–2 business days if we extract DNA from your bacterial cell pellet.
Hybrid Sequencing (ONT + Illumina)
• 6–8 business days for hybrid assembly, or 8–10 business days if we also perform in-house DNA extraction. • Perfect for applications requiring ultra-accurate SNP detection or polished assemblies to resolve tricky homopolymers.
Turnaround Times & Workflow Options
Pre-Extracted gDNA
1 business day from receipt of samples. Ideal if you already have purified, high-quality DNA.
In-House gDNA Extraction
1–2 business days if we extract DNA from your bacterial cell pellet.
Hybrid Sequencing (ONT + Illumina)
• 6–8 business days for hybrid assembly, or 8–10 business days if we also perform in-house DNA extraction. • Perfect for applications requiring ultra-accurate SNP detection or polished assemblies to resolve tricky homopolymers.
Turnaround Times & Workflow Options
Pre-Extracted gDNA
1 business day from receipt of samples. Ideal if you already have purified, high-quality DNA.
In-House gDNA Extraction
1–2 business days if we extract DNA from your bacterial cell pellet.
Hybrid Sequencing (ONT + Illumina)
• 6–8 business days for hybrid assembly, or 8–10 business days if we also perform in-house DNA extraction. • Perfect for applications requiring ultra-accurate SNP detection or polished assemblies to resolve tricky homopolymers.
Data & File Types Delivered
Data & File Types
Delivered
FASTA files
.fna (contig nucleotide sequences) = polished consensus sequence of the genome
.faa (protein amino acid sequences)
.ffn (gene nucleotide sequences)
GenBank files
.gbff (annotated contig sequences) = polished and annotated consensus sequence of the genome
Report
.html = An analytical report of the key metrics for the assembly (including completeness of the assembly based on CheckM and general species identification of the contigs)
FASTQ files
.fastq.gz = a compressed file of all the raw ONT sequencing reads
Various other Bakta annotation files
Various other Bakta annotation files
How it works
How it works
How it works
Preparing your Bacterial gDNA Samples
Preparing your Bacterial gDNA Samples
Preparing your Bacterial gDNA Samples
This service requires 1 µg of gDNA, at a concentration of 50 ng/µL in 20 µL of elution buffer NOTE: The Hybrid Sequencing service requires 1.5μg in 30μL of elution buffer.
Step 1
Prepare a clonal culture of your bacteria
Prepare a clonal culture of your bacteria
Prepare a clonal culture of your bacteria
Step 2
Extract and purify gDNA from your clonal bacterial culture. Any extraction method that yields high quality, high purity, high molecular weight (HMW), double-stranded gDNA that is free of nicks, gaps, breaks, and contaminants is suitable for this sequencing service.
Extract and purify gDNA from your clonal bacterial culture. Any extraction method that yields high quality, high purity, high molecular weight (HMW), double-stranded gDNA that is free of nicks, gaps, breaks, and contaminants is suitable for this sequencing service.
Extract and purify gDNA from your clonal bacterial culture. Any extraction method that yields high quality, high purity, high molecular weight (HMW), double-stranded gDNA that is free of nicks, gaps, breaks, and contaminants is suitable for this sequencing service.
Step 3
Perform QC to verify your gDNA quantity, quality, and purity. We recommended >50% of the DNA above 15 kb in length and recommended purity of 260/280 above 1.8 and 260/230 between 2.0-2.2. See FAQ section for tips and tricks on how best practices to QC your gDNA. See FAQ section for best practices to QC your gDNA.
Preparing your Bacterial Cell Pellets
Preparing your Bacterial Cell Pellets
All samples must be sent as cell pellets that have been resuspended in Zymo 1X DNA/RNA Shield preservative. Please do not use any other type of preservation media. We accept cell pellets from both BSL1 and BSL2 strains.
We recommend growing a fresh clonal culture of your bacteria in liquid broth and harvesting the cells when they are in exponential growth or early stationary phase. See FAQ section for further instructions on preparing bacterial cell pellets for extraction.
All samples must be sent as cell pellets that have been resuspended in Zymo 1X DNA/RNA Shield preservative. Please do not use any other type of preservation media. We accept cell pellets from both BSL1 and BSL2 strains.
We recommend growing a fresh clonal culture of your bacteria in liquid broth and harvesting the cells when they are in exponential growth or early stationary phase. See FAQ section for further instructions on preparing bacterial cell pellets for extraction.
All samples must be sent as cell pellets that have been resuspended in Zymo 1X DNA/RNA Shield preservative. Please do not use any other type of preservation media. We accept cell pellets from both BSL1 and BSL2 strains.
We recommend growing a fresh clonal culture of your bacteria in liquid broth and harvesting the cells when they are in exponential growth or early stationary phase. See FAQ section for further instructions on preparing bacterial cell pellets for extraction.
Ideal Applications
Ideal Applications
Ideal Applications
Gene Detection & Arrangement
Gene Detection & Arrangement
Confirm the presence and orientation of vital genes—including virulence factors and AMR markers.
Strain Typing & Species Identification
Strain Typing & Species Identification
Identify the exact organism you’re working with—even if you’re unsure at the start.
De Novo Assembly of Novel Microbes
De Novo Assembly of Novel Microbes
Sequence new or poorly characterized species with full, contiguous assemblies.
Mobile Element & Phage Screening
Mobile Element & Phage Screening
Detect plasmids, transposons, and bacteriophages missed by short-read methods.
FAQ
FAQ
FAQ
How do you sequence bacterial genome samples?
How do you sequence bacterial genome samples?
How do you sequence bacterial genome samples?
How do you sequence bacterial genome samples?
How is the bacterial genome assembly generated?
How is the bacterial genome assembly generated?
How is the bacterial genome assembly generated?
How is the bacterial genome assembly generated?
What data & file types will I receive for successful bacterial genome sequencing?
What data & file types will I receive for successful bacterial genome sequencing?
What data & file types will I receive for successful bacterial genome sequencing?
What data & file types will I receive for successful bacterial genome sequencing?
Can you identify the species if I don’t know it beforehand?
Can you identify the species if I don’t know it beforehand?
Can you identify the species if I don’t know it beforehand?
Can you identify the species if I don’t know it beforehand?
Will I get a fully closed genome?
Will I get a fully closed genome?
Will I get a fully closed genome?
Will I get a fully closed genome?
Is raw data included?
Is raw data included?
Is raw data included?
Is raw data included?
Can you confirm CRISPR edits or gene placements?
Can you confirm CRISPR edits or gene placements?
Can you confirm CRISPR edits or gene placements?
Can you confirm CRISPR edits or gene placements?
How do I get my results?
How do I get my results?
How do I get my results?
How do I get my results?
What data targets do you use for the Oxford Nanopore Sequencing Service?
What data targets do you use for the Oxford Nanopore Sequencing Service?
What data targets do you use for the Oxford Nanopore Sequencing Service?
What data targets do you use for the Oxford Nanopore Sequencing Service?
What data targets do you use for the Oxford Nanopore Sequencing Service?
What data targets do you use for the Oxford Nanopore Sequencing Service?
What data targets do you use for the Hybrid (Oxford Nanopore + Illumina) Sequencing Service?
What data targets do you use for the Hybrid (Oxford Nanopore + Illumina) Sequencing Service?
What data targets do you use for the Hybrid (Oxford Nanopore + Illumina) Sequencing Service?
What data targets do you use for the Hybrid (Oxford Nanopore + Illumina) Sequencing Service?
How do I prepare a clonal culture of bacteria?
How do I prepare a clonal culture of bacteria?
How do I prepare a clonal culture of bacteria?
How do I prepare a clonal culture of bacteria?
How do I extract and purify gDNA from my clonal bacterial culture?
How do I extract and purify gDNA from my clonal bacterial culture?
How do I extract and purify gDNA from my clonal bacterial culture?
How do I extract and purify gDNA from my clonal bacterial culture?
How do I prepare my bacterial cell pellets for extraction?
How do I prepare my bacterial cell pellets for extraction?
How do I prepare my bacterial cell pellets for extraction?
How do I prepare my bacterial cell pellets for extraction?
Does your extraction service include growing cultures prior to extraction?
Does your extraction service include growing cultures prior to extraction?
Does your extraction service include growing cultures prior to extraction?
Does your extraction service include growing cultures prior to extraction?
Do you perform any QC on my gDNA prior to sequencing?
Do you perform any QC on my gDNA prior to sequencing?
Do you perform any QC on my gDNA prior to sequencing?
Do you perform any QC on my gDNA prior to sequencing?
How should I QC my gDNA prior to submitting my sample?
How should I QC my gDNA prior to submitting my sample?
How should I QC my gDNA prior to submitting my sample?
How should I QC my gDNA prior to submitting my sample?
How should I handle gDNA samples?
How should I handle gDNA samples?
How should I handle gDNA samples?
How should I handle gDNA samples?
What guarantees do you provide?
What guarantees do you provide?
What guarantees do you provide?
What guarantees do you provide?
What is your policy when bacterial genome sequencing fails?
What is your policy when bacterial genome sequencing fails?
What is your policy when bacterial genome sequencing fails?
What is your policy when bacterial genome sequencing fails?