ZeroPrep Colony Sequencing
ZeroPrep Colony Sequencing
ZeroPrep Colony Sequencing

Go straight from colony to whole plasmid sequence, overnight. No primers or DNA prep needed, for $20 per sample.

Go straight from colony to whole plasmid sequence, overnight. No primers or DNA prep needed, for $20 per sample.

Go straight from colony to whole plasmid sequence, overnight. No primers or DNA prep needed, for $20 per sample.

Order Now

Go straight from colony to whole plasmid sequence, overnight. No primers or DNA prep needed, for $20 per sample.

Order Now

Accelerate your research

Accelerate your research

No primers, no miniprep, with results in your inbox in less than a day. Compress multiple cloning cycles into a single workweek.

Conquer challenging plasmids

Conquer challenging plasmids

Conquer challenging plasmids

Conquer challenging plasmids

Sequence directly from bacterial colonies, even when working with low-copy plasmids.

Get all the answers

Get all the answers

Receive a fully annotated plasmid map, consensus sequence, raw reads, and key quality metrics (chromatogram, coverage plots, and more).

Accelerate your research

No primers, no miniprep, with results in your inbox in less than a day. Compress multiple cloning cycles into a single workweek.

Conquer challenging plasmids

Sequence directly from bacterial colonies, even when working with low-copy plasmids.

Get all the answers

Receive a fully annotated plasmid map, consensus sequence, raw reads, and key quality metrics (chromatogram, coverage plots, and more).

Goodbye minipreps, hello answers

Goodbye minipreps, hello answers

Goodbye minipreps, hello answers

Goodbye minipreps, hello answers

Are you tired of performing minipreps to purify plasmids? Want to reclaim the time minipreps take, while also cutting out the cost of miniprep supplies?  Plasmidsaurus ZeroPrep Colony Sequencing is a direct colony sequencing service that eliminates the need for plasmid DNA isolation. Researchers can submit bacterial colonies and receive assembled plasmid sequences within one day. This service produces a consensus sequence of the same quality as our Whole Plasmid Sequencing service.

Why ZeroPrep Avoids Rolling Circle Amplification (RCA)

Why ZeroPrep Avoids Rolling Circle Amplification (RCA)

RCA often introduces coverage biases and can lead to assembly errors by over- or under-amplifying regions of the plasmid. It also adds extra steps that slow down turnaround. By skipping RCA, ZeroPrep maintains an amplification-free workflow, delivering more uniform coverage and faster results.

ZeroPrep saves time so you can focus on your science

ZeroPrep saves time so you can focus on your science

ZeroPrep saves time so you can focus on your science

ZeroPrep saves time so you can focus on your science

Academic groups

Academic groups

exploring many plasmid variants simultaneously

Biotech & pharma

Biotech & pharma

gene therapy workflows, CRISPR libraries, viral vector backbones

Any lab

Any lab

wanting to skip the headache and expense of manual plasmid preps

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"ZeroPrep saved us 2–3 days per cloning cycle. We can now finish cloning and validation in a single week."

"ZeroPrep saved us 2–3 days per cloning cycle. We can now finish cloning and validation in a single week."

"ZeroPrep saved us 2–3 days per cloning cycle. We can now finish cloning and validation in a single week."

Anonymous Scientist at a gene therapy company

Anonymous Scientist at a gene therapy company

Anonymous Scientist at a gene therapy company

Anonymous Scientist at a gene therapy company

Anonymous Scientist at a gene therapy company

Data

Data

Data

Data

Figure 1. Comparison of the same low-copy plasmid prepared by conventional miniprep (left) vs. ZeroPrep (right). Each read length distribution confirms an abundance of full-length reads, resulting in identical plasmid assemblies and high-confidence coverage in both cases.

How it works

Step 1

Prepare your samples – choose from three options

  • Straight from the plate: Circled and numbered colonies, shipped on agar plates.

  • Resuspended colonies: 1 large colony resuspended in 10 uL of water, shipped in 200 μl strip tubes.

  • Liquid culture: 100 μl of liquid culture, shipped in 200 μl strip tubes.

Step 2

Place your order and drop off your samples

Place your order online, then drop off your sample at a dropbox or ship it with a printed label.

Step 3

Get results overnight

Get results overnight

Your results will be waiting in your inbox the next morning.

How it works

Step 1

  • Straight from the plate: Circled and numbered colonies, shipped on agar plates.

  • Resuspended colonies: 1 large colony resuspended in 10 uL of water, shipped in 200 μl strip tubes.

  • Liquid culture: 100 μl of liquid culture, shipped in 200 μl strip tubes.

Prepare your samples – choose from three options

Step 2

Place your order online, then drop off your sample at a dropbox or ship it with a printed label.

Place your order and drop off your samples

Step 3

Your results will be waiting in your inbox the next morning.

Get results overnight

How it works

Step 1

Prepare your samples – choose from three options

  • Straight from the plate: Circled and numbered colonies, shipped on agar plates.

  • Resuspended colonies: 1 large colony resuspended in 10 uL of water, shipped in 200 μl strip tubes.

  • Liquid culture: 100 μl of liquid culture, shipped in 200 μl strip tubes.

Step 2

Place your order online, then drop off your sample at a dropbox or ship it with a printed label.

Place your order and drop off your samples

Step 3

Your results will be waiting in your inbox the next morning.

Get results overnight

Data & File Types Delivered

Data & File Types
Delivered

Data & File Types Delivered

Data & File Types
Delivered

Concensus sequence

.fasta: Polished plasmid sequence

.gbk: Polished sequence with annotations

Plasmid Map (.html)

Interactive map

Read Length Histogram (.png)

Distribution of raw read lengths

Virtual Gel (.png)

Visual “gel” of read lengths per sample

Chromatogram (.ab1)

Chromatogram (.ab1)

Chromatogram (.ab1)

Nucleotide abundance across the consensus

Coverage Plot (.png)

Coverage depth at each plasmid position

Per-Base Data (.txt / .tsv)

Alignment stats, multimer analysis, composition, and contamination

Raw Reads (.fastq.gz)

Sequences of aligned reads (optional download)

Learn more from our  Results Interpretation Guide

Learn more from our  Results Interpretation Guide

Learn more from our  Results Interpretation Guide

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FAQ

FAQ

FAQ

FAQ

How do you sequence plasmid & other circular samples?

How do you sequence plasmid & other circular samples?

How do you sequence plasmid & other circular samples?

How do you sequence plasmid & other circular samples?

How do you sequence plasmid & other circular samples?

How accurate are the plasmid sequencing results?

How accurate are the plasmid sequencing results?

How accurate are the plasmid sequencing results?

How accurate are the plasmid sequencing results?

How accurate are the plasmid sequencing results?

What data & file types will I receive for successful plasmid sequencing?

What data & file types will I receive for successful plasmid sequencing?

What data & file types will I receive for successful plasmid sequencing?

What data & file types will I receive for successful plasmid sequencing?

What data & file types will I receive for successful plasmid sequencing?

What is your policy when plasmid samples fail?

What is your policy when plasmid samples fail?

What is your policy when plasmid samples fail?

What is your policy when plasmid samples fail?

What is your policy when plasmid samples fail?

Why doesn’t ZeroPrep use RCA (rolling circle amplification)?

Why doesn’t ZeroPrep use RCA (rolling circle amplification)?

Why doesn’t ZeroPrep use RCA (rolling circle amplification)?

Why doesn’t ZeroPrep use RCA (rolling circle amplification)?

Why doesn’t ZeroPrep use RCA (rolling circle amplification)?

Will you grow cells overnight if I send in LB media?

Will you grow cells overnight if I send in LB media?

Will you grow cells overnight if I send in LB media?

Will you grow cells overnight if I send in LB media?

Will you grow cells overnight if I send in LB media?