Are you tired of performing minipreps to purify plasmids? Want to reclaim the time minipreps take, while also cutting out the cost of miniprep supplies? Plasmidsaurus ZeroPrep Colony Sequencing is a direct colony sequencing service that eliminates the need for plasmid DNA isolation. Researchers can submit bacterial colonies and receive assembled plasmid sequences within one day. This service produces a consensus sequence of the same quality as our Whole Plasmid Sequencing service.
RCA often introduces coverage biases and can lead to assembly errors by over- or under-amplifying regions of the plasmid. It also adds extra steps that slow down turnaround. By skipping RCA, ZeroPrep maintains an amplification-free workflow, delivering more uniform coverage and faster results.
exploring many plasmid variants simultaneously
gene therapy workflows, CRISPR libraries, viral vector backbones
wanting to skip the headache and expense of manual plasmid preps
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Figure 1. Comparison of the same low-copy plasmid prepared by conventional miniprep (left) vs. ZeroPrep (right). Each read length distribution confirms an abundance of full-length reads, resulting in identical plasmid assemblies and high-confidence coverage in both cases.
Concensus sequence
.fasta: Polished plasmid sequence
.gbk: Polished sequence with annotations
Plasmid Map (.html)
Interactive map
Read Length Histogram (.png)
Distribution of raw read lengths
Virtual Gel (.png)
Visual “gel” of read lengths per sample
Nucleotide abundance across the consensus
Coverage Plot (.png)
Coverage depth at each plasmid position
Per-Base Data (.txt / .tsv)
Alignment stats, multimer analysis, composition, and contamination
Raw Reads (.fastq.gz)
Sequences of aligned reads (optional download)
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