How it works
How it works
How it works
How it works
Step 1
Prepare your samples
Prepare your samples
Prepare your samples
Prepare your samples
Send us purified amplicons from the 16S, 18S, and/or ITS marker genes, amplified at your lab using PCR primers for the full-length genes. Each sample you submit may contain one or more of these three amplified marker genes from a given biological specimen.
Submit your final purified DNA amplicons normalized to 10-20 ng/uL in elution buffer (10 mM Tris, pH 8.5) or nuclease-free water.
Send us purified amplicons from the 16S, 18S, and/or ITS marker genes, amplified at your lab using PCR primers for the full-length genes. Each sample you submit may contain one or more of these three amplified marker genes from a given biological specimen.
Submit your final purified DNA amplicons normalized to 10-20 ng/uL in elution buffer (10 mM Tris, pH 8.5) or nuclease-free water.
Send us purified amplicons from the 16S, 18S, and/or ITS marker genes, amplified at your lab using PCR primers for the full-length genes. Each sample you submit may contain one or more of these three amplified marker genes from a given biological specimen.
Submit your final purified DNA amplicons normalized to 10-20 ng/uL in elution buffer (10 mM Tris, pH 8.5) or nuclease-free water.
Step 2
Place your order and drop off your samples
Place your order and drop off your samples
Place your order and drop off your samples
Place your order online, then drop off your sample at a dropbox or ship it with a printed label.
Place your order online, then drop off your sample at a dropbox or ship it with a printed label.
Place your order online, then drop off your sample at a dropbox or ship it with a printed label.
Step 3
Get results overnight
Get results overnight
Get results overnight
The next morning you’ll receive an email to download your results from your Dashboard.
The next morning you’ll receive an email to download your results from your Dashboard.
The next morning you’ll receive an email to download your results from your Dashboard.
Data and File Types Delivered
Data and File Types Delivered
Raw read sequences (.fastq.gz file)
Data and File Types Delivered
Data and File Types Delivered
Provides the sequences of all raw reads produced by your sample (up to 5,000 raw reads for Standard service, 10,000 raw reads for Big, and 20,000 raw reads for Huge)
Raw read sequences (.fastq.gz file)
Provides the sequences of all raw reads produced by your sample (up to 5,000 raw reads for Standard service, 10,000 raw reads for Big, and 20,000 raw reads for Huge)
Raw read sequences (.fastq.gz file)
Provides the sequences of all raw reads produced by your sample (up to 5,000 raw reads for Standard service, 10,000 raw reads for Big, and 20,000 raw reads for Huge)
Taxonomy table (.csv file)
Taxonomy table (.csv file)
Lists all taxa found in your sample that are above a relative threshold of 0.1% abundance. The list includes columns with the NCBI taxonomy ID, relative abundance, and full taxonomic ranks for each taxa from kingdom to species.
Stats (.csv file)
Stats (.csv file)
Stats (.csv file)
Lists statistics about the sequencing reads (total number reads, total number bases, read quality, etc.)
Taxonomy plot by genus (.png file)
Taxonomy plot by genus (.png file)
Taxonomy plot by genus (.png file)
Shows the relative abundance of the top 20 genera in your sample.
Taxonomy plot by species (.png file)
Taxonomy plot by species (.png file)
Taxonomy plot by species (.png file)
Shows the relative abundance of the top 20 species in your sample.
Read length histogram (.png file)
Read length histogram (.png file)
Read length histogram (.png file)
Displays the read lengths of all raw reads produced by the sample, including coloration to indicate the kingdom that the reads originate from (bacteria, archaea, protists, fungi, etc.)
FAQ
FAQ
FAQ
FAQ
How do I ensure the best chance of successful sequencing?
How do I ensure the best chance of successful sequencing?
How do I ensure the best chance of successful sequencing?
How do I ensure the best chance of successful sequencing?
How do I ensure the best chance of successful sequencing?
Do I need to provide sequencing primers with my amplicons?
Do I need to provide sequencing primers with my amplicons?
Do I need to provide sequencing primers with my amplicons?
Do I need to provide sequencing primers with my amplicons?
Do I need to provide sequencing primers with my amplicons?
How do I amplify & purify my target genes?
How do I amplify & purify my target genes?
How do I amplify & purify my target genes?
How do I amplify & purify my target genes?
How do I amplify & purify my target genes?
How do I verify concentration, quality & purity?
How do I verify concentration, quality & purity?
How do I verify concentration, quality & purity?
How do I verify concentration, quality & purity?
How do I verify concentration, quality & purity?
How do you sequence and analyze my samples?
How do you sequence and analyze my samples?
How do you sequence and analyze my samples?
How do you sequence and analyze my samples?
How do you sequence and analyze my samples?
How is taxonomic analysis performed for Microbiome Amplicon samples?
How is taxonomic analysis performed for Microbiome Amplicon samples?
How is taxonomic analysis performed for Microbiome Amplicon samples?
How is taxonomic analysis performed for Microbiome Amplicon samples?
How is taxonomic analysis performed for Microbiome Amplicon samples?
How many reads do I need?
How many reads do I need?
How many reads do I need?
How many reads do I need?
How many reads do I need?
What is the limit of detection for the lowest abundance community members that can be detected?
What is the limit of detection for the lowest abundance community members that can be detected?
What is the limit of detection for the lowest abundance community members that can be detected?
What is the limit of detection for the lowest abundance community members that can be detected?
What is the limit of detection for the lowest abundance community members that can be detected?
What is your turnaround time for returning Microbiome Amplicon sequencing results?
What is your turnaround time for returning Microbiome Amplicon sequencing results?
What is your turnaround time for returning Microbiome Amplicon sequencing results?
What is your turnaround time for returning Microbiome Amplicon sequencing results?
What is your turnaround time for returning Microbiome Amplicon sequencing results?
How do I get my results?
How do I get my results?
How do I get my results?
How do I get my results?
How do I get my results?
How do I amplify my target genes for the Microbiome Amplicon service?
How do I amplify my target genes for the Microbiome Amplicon service?
How do I amplify my target genes for the Microbiome Amplicon service?
How do I amplify my target genes for the Microbiome Amplicon service?
How do I amplify my target genes for the Microbiome Amplicon service?
Can I submit non-full length amplicons for sequencing, e.g. amplicons generated from only the V4 variable region of the 16S gene?
Can I submit non-full length amplicons for sequencing, e.g. amplicons generated from only the V4 variable region of the 16S gene?
Can I submit non-full length amplicons for sequencing, e.g. amplicons generated from only the V4 variable region of the 16S gene?
Can I submit non-full length amplicons for sequencing, e.g. amplicons generated from only the V4 variable region of the 16S gene?
Can I submit non-full length amplicons for sequencing, e.g. amplicons generated from only the V4 variable region of the 16S gene?
What guarantees do you provide?
What guarantees do you provide?
What guarantees do you provide?
What guarantees do you provide?
What guarantees do you provide?
Why did my Microbiome Amplicon sample fail?
Why did my Microbiome Amplicon sample fail?
Why did my Microbiome Amplicon sample fail?
Why did my Microbiome Amplicon sample fail?
Why did my Microbiome Amplicon sample fail?
Can you amplify the 16S gene for me?
Can you amplify the 16S gene for me?
Can you amplify the 16S gene for me?
Can you amplify the 16S gene for me?
Can you amplify the 16S gene for me?
I have suggestions about the types of reports and figures you should generate from Microbiome amplicon results, or which databases you should use for processing the data. How can I let you know?
I have suggestions about the types of reports and figures you should generate from Microbiome amplicon results, or which databases you should use for processing the data. How can I let you know?
I have suggestions about the types of reports and figures you should generate from Microbiome amplicon results, or which databases you should use for processing the data. How can I let you know?
I have suggestions about the types of reports and figures you should generate from Microbiome amplicon results, or which databases you should use for processing the data. How can I let you know?
I have suggestions about the types of reports and figures you should generate from Microbiome amplicon results, or which databases you should use for processing the data. How can I let you know?
How it works
Step 1
Prepare your samples
Send us purified genomic DNA that has been extracted at your lab from the source material (soil, swabs, feces. etc.).
Submit your final purified metagenomic DNA normalized to 10ng/uL in elution buffer (10 mM Tris, pH 8.5) or nuclease-free water.
Step 2
Place your order and drop off your samples
Place your order online, then drop off your sample at a dropbox or ship it with a printed label.
Step 3
Get results overnight
You’ll receive a full report with de novo assemblies and the ability to download raw reads for your own analysis.
